Thermo Scientific Pierce Protein Precipitation Microplate 96 Well 8 Plates

Thermo Scientific Pierce Protein Precipitation Plates - 96-Well Format, 2 mL Well Volume, Graded Hydrophobic Frit, 0.2 Micron Cutoff Membrane (MPN: 90037)

The Thermo Scientific 90037 (part of the Pierce Biotechnology product family) is a high-performance 96-well protein precipitation microplate engineered for rapid, automated, or manual extraction and clarification of small molecule analytes from complex biological fluids like plasma, serum, and whole blood. Featuring a spacious 2 mL well capacity that accommodates serum sample volumes ranging from 15 to 400 microliters, these solvent-inert polypropylene plates utilize a specialized "protein crash" workflow technique. Built with a leach-free, graded hydrophobic frit system, the plate retains organic solvents like acetonitrile or methanol without dripping during critical automated platform shaking loops, then allows purified filtrates to be drawn down while trapping precipitated protein aggregates behind a clean 0.2 micron cutoff membrane.

Technical Performance and Design

• Drip-Free Hydrophobic Frit Design: Pre-packed with a non-wetting, graded hydrophobic frit matrix. This specialized barrier completely retains aggressive organic solvents (including 100 percent acetonitrile or methanol) for up to four hours without leaking or dripping, allowing for long on-plate mixing and precipitation routines.

• Anti-Clogging 0.2 Micron Cutoff Layer: Outfitted with a graded filter system featuring decreasing pore sizes that lead down to a tight 0.2 micron cutoff membrane. This layout locks away dense, sticky precipitated protein shards and macromolecular debris without plugging up, guaranteeing high eluate filtration flow and analyte recovery.

• Oversized 2 mL Processing Cavities: Well dimensions support a generous 2 mL volume profile, which is highly optimized for executing standard 3:1 or 4:1 solvent-to-serum precipitation ratios without well overflowing or side-wall fluid aerosol talk during intensive platform mixing passes.

• Multi-System Elution Flexibility: The structural bottom profile of the plate deck is engineered to release clean filtrates under uniform mechanical forces, operating with standard laboratory vacuum manifolds, automated positive pressure modules, or standard multiwell plate centrifuge buckets.

Product Specifications

• Catalog Number / MPN: 90037 (Commonly cross-listed as PI90037)

• Manufacturer / Brand: Thermo Scientific / Pierce Protein lines

• Format Layout: Standard 96-Well Grid (8 x 12 Array Arrangement)

• Total Well Capacity: 2.0 mL total volume per well

• Recommended Sample Input: 15 to 400 microliters of serum/plasma per well

• Filter Membrane Profile: Graded Hydrophobic Frit terminating at a 0.2 micron microfiltration membrane

• Material Composition: Solvent-Inert Medical-Grade Polypropylene (PP)

• Sterility / Process State: Non-Sterile (Optimized for biofluid small molecule isolation and HPLC sample cleanup tasks)

• Included Accessories: Every factory kit package ships complete with specialized plate sealing covers

Key Benefits and Applications

• LC-MS and HPLC Sample Prep: The industry-standard microplate tool used to strip out background protein interferences from blood derivatives, preparing clean small-molecule extracts for analytical mass spectrometry injections in 10 to 30 minutes.

• Pharmacokinetic and RED Assay Cleanup: Highly recommended for downstream sample harvesting from Rapid Equilibrium Dialysis (RED) devices, supporting essential plasma-protein binding (PPB) evaluations during drug candidate profiling phases.

• High-Throughput Automation Workflows: Conforms strictly to ANSI/SLAS microplate footprints, allowing automated liquid handling arms, plate shakers, and robotic vacuum stations to grip and process plates without custom adjustments.

• Zero Extractable Sample Protection: Constructed using leach-free polymer materials, ensuring no baseline plasticizer compounds or slip-agents bleed into the eluted samples to disrupt high-sensitivity chromatography signals.

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