Thermo Scientific Slide-A-Lyzer Cassette 0.5 ml 3.5K MWCO 9 Cassettes

Thermo Scientific 87722 Slide-A-Lyzer G2 Dialysis Cassette – 0.5 mL, 3.5K MWCO

The Thermo Scientific 87722 is a Slide-A-Lyzer G2 Dialysis Cassette engineered for the rapid, high-recovery dialysis of small-volume biological samples. Designed to accommodate up to 0.5 mL of fluid, this ready-to-use cassette completely eliminates the tedious, leak-prone process of knotting traditional dialysis tubing. It features a low-binding regenerated cellulose membrane with a strict 3,500 Dalton Molecular Weight Cut-Off (3.5K MWCO), ensuring that small peptides and target proteins are securely retained while unwanted salts, crosslinkers, and small molecular contaminants diffuse freely into the exchange buffer. The G2 architecture incorporates built-in air chambers for self-floating buoyancy and a self-sealing syringe port for uncompromised sample loading and retrieval.

Technical Performance and Design

• Regenerated Cellulose Membrane: The 3.5K MWCO membrane provides exceptional chemical compatibility and extremely low non-specific protein binding, ensuring maximum yield of dilute or highly valuable macromolecules.

• Self-Sealing Syringe Port: Researchers can safely load and extract their sample using a standard laboratory syringe and needle, entirely bypassing the sample loss associated with clamping or knotting bulk tubing.

• Maximized Surface Area: The flat, dual-membrane rigid frame maximizes the surface-area-to-volume ratio, significantly accelerating the diffusion rate and reducing total dialysis time to just a few hours.

• Self-Floating Architecture: The integrated air chambers ensure the cassette floats vertically in the dialysis buffer without the need for cumbersome external buoys or flotation rings.

Product Specifications

• Catalog Number: 87722

• Product Line: Slide-A-Lyzer G2

• Volume Capacity: 0.5 mL

• Molecular Weight Cut-Off (MWCO): 3.5 kDa (3,500 Daltons)

• Membrane Material: Regenerated Cellulose

• Packaging: Box of 10 Cassettes

Key Benefits & Applications

• Peptide & Small Protein Recovery: The tight 3.5K MWCO is specifically chosen for dialyzing low-molecular-weight proteins and peptides that would otherwise escape through standard 10K MWCO membranes.

• Buffer Exchange & Desalting: The standard choice for rapidly removing imidazole, reducing agents, or high salt concentrations from purified fractions prior to downstream functional assays or lyophilization.

• High Volumetric Recovery: The rigid internal geometry allows users to withdraw the sample with a syringe needle without puncturing the opposite membrane, routinely delivering macromolecule recovery rates exceeding 95%.

• Workflow Efficiency: Arrives cleanroom-assembled and ready to use, completely bypassing the hours of boiling and chemical pretreatment required for rolls of dry dialysis tubing.

All product names, logos, and brands are property of their respective owners.