Thermo Scientific Slide-A-Lyzer Mini Unit 100 ul 10K MWCO Box of 10 Units

Thermo Scientific 69574 Slide-A-Lyzer Mini Dialysis Units – 10K MWCO, 0.1 mL Capacity

The Thermo Scientific 69574 is a high-performance micro-dialysis device designed for the rapid and efficient processing of low-volume biological samples. Each unit features a 10,000 MWCO (Molecular Weight Cut-Off) regenerated cellulose membrane, making it ideal for the removal of salts, detergents, and low-molecular-weight contaminants from proteins or nucleic acids greater than 10 kDa. Designed for sample volumes between 10 µL and 100 µL, the 69574 utilizes a unique cup-shaped geometry that maximizes surface-area-to-volume ratio, enabling complete dialysis in as little as 4 to 8 hours. These units are designed to be used with a floating buoy (sold separately) in a buffer reservoir, ensuring the membrane remains submerged for optimal diffusion. The convenient "pipette-in, pipette-out" design ensures virtually total sample recovery, eliminating the risk of loss associated with traditional dialysis tubing or centrifugal filters.

Technical Performance and Design

• 10K MWCO Regenerated Cellulose: Provides a sharp cut-off and low non-specific binding, ensuring high recovery of proteins and peptides while effectively diffusing out smaller contaminating molecules.

• Micro-Volume Optimization: Specifically engineered for the 10–100 µL range, preventing the sample dilution that often occurs in larger dialysis devices.

• High Surface Area: The integrated membrane at the base of the cup provides a large diffusion area relative to the small sample volume, significantly accelerating the kinetics of buffer exchange.

• Leak-Proof Construction: The membrane is ultrasonically welded to the polypropylene housing, ensuring a secure seal that prevents sample leakage into the external buffer bath.

Product Specifications

• Catalog Number: 69574

• MWCO: 10,000 Daltons (10 kDa)

• Capacity: 10 µL – 100 µL

• Membrane Type: Low-binding Regenerated Cellulose

• Quantity: Typically sold in packs of 50 units

• Format: Individual cup-style units

Key Benefits & Applications

• Protein Desalting & Buffer Exchange: The standard method for preparing small protein samples for mass spectrometry, NMR, or isoelectric focusing by removing interfering salts and urea.

• Detergent Removal: Effectively removes SDS, Triton X-100, and other surfactants used during protein extraction that may interfere with downstream assays.

• Radiolabeling Cleanup: Rapidly separates unreacted radioisotopes or fluorescent dyes from labeled macromolecules, ensuring high signal-to-noise ratios in detection assays.

• Ease of Use: Requires no knots, clamps, or syringes; samples are simply pipetted into the unit, which is then placed in a float for dialysis, and recovered via pipette upon completion.

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