Thermo Scientific Top Works for Nalgene Carboy - 3 Aseptic Closure Systems

Thermo Scientific Nalgene Top Works™ Flexible System (53B Closure, Catalog 2135-5303)

The Thermo Scientific Nalgene Top Works™ 2135-5303 is a premium, "plug-and-play" closure assembly designed to create an instant aseptic fluid transfer system. Unlike standard ported caps that require manual tubing insertion, this system features platinum-cured silicone tubing fused directly into a thermoplastic elastomer (TPE) stopper insert. This "one-piece" integrated construction eliminates the risk of tubing slippage or bypass leakage at the insertion point, providing a significantly higher level of sterility assurance for critical upstream applications. Engineered to fit vessels with a 53B (53 mm) neck finish, this assembly is the standard choice for smaller Nalgene carboys and heavy-duty vacuum bottles.

Technical Performance and Design

• Fused One-Piece Construction: The silicone tubing is permanently bonded to the TPE stopper, creating a seamless, leakproof interface that maintains its integrity even under the thermal stress of repeated autoclaving.

• Versatile 3-Port Configuration: Features a specialized tri-port layout designed for simultaneous fluid management: two high-flow 1/4-inch (6.4 mm) ID lines for inlet/outlet streams and one 1/8-inch (3.2 mm) ID line for venting or sampling.

• Platinum-Cured Silicone Tubing: Utilizes high-purity, ultra-flexible silicone that ensures low extractables and minimal binding of proteins or active pharmaceutical ingredients (APIs).

• Leakproof 53B Interface: Designed with a precision-molded polypropylene screw cap that provides a secure, airtight seal on standard 53 mm neck finishes, making it ideal for vacuum or pressure-driven transfers.

Product Specifications

• Catalog Number: 2135-5303

• Closure Size: 53B (53 mm)

• Port 1 & 2: 1/4-inch (6.4 mm) ID Tubing

• Port 3: 1/8-inch (3.2 mm) ID Tubing

• Materials: Polypropylene (Cap), TPE (Stopper), Platinum-Cured Silicone (Tubing)

• Sterility/Sanitation: Non-sterile; Fully Autoclavable (121°C, 15 psi for 20 min)

• Regulatory: USP Class VI compliant; Non-cytotoxic; Food-grade (CFR 21.177)

Key Benefits & Applications

• Aseptic Inoculation: Facilitates the sterile transfer of seed cultures into larger bioreactors while maintaining a closed system to prevent environmental contamination.

• Media & Buffer Transfer: The two 1/4" lines allow for efficient, high-volume fluid movement, while the 1/8" line is ideal for attaching a 0.2 µm vent filter to maintain pressure equilibrium.

• Closed-System Sampling: Allows for the removal of small-volume samples through the dedicated 1/8" line without disrupting the main process flow or exposing the vessel contents.

• Plug-and-Play Integration: Eliminates the labor-intensive process of fabricating custom carboy caps, providing a validated, off-the-shelf solution that reduces the risk of operator error.

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