Waters RapiGest SF Surfactant 10 mg

Waters 186002123 RapiGest SF Surfactant – 10 mg

The Waters 186002123 is a 10 mg vial of RapiGest SF, a patented, acid-cleavable surfactant specifically engineered to enhance the in-solution enzymatic digestion of proteins for liquid chromatography-mass spectrometry (LC-MS) analysis. Unlike traditional denaturants such as SDS (Sodium Dodecyl Sulfate) or urea, which severely suppress mass spectrometry signals and require rigorous sample cleanup prior to injection, RapiGest SF is designed to completely degrade at low pH. This allows researchers to rapidly solubilize complex protein mixtures, unfold tightly packed domains to expose cleavage sites to enzymes like trypsin, and then easily remove the surfactant via acid precipitation without losing precious peptides. It is an industry standard in shotgun proteomics, membrane protein characterization, and targeted biomarker discovery.

Technical Performance and Design

• Acid-Cleavable Chemistry: The surfactant remains fully intact and stable at the neutral to slightly alkaline pH required for optimal trypsin digestion (pH 7–8). However, when the reaction is quenched with acid (dropping the pH below 2.0), it rapidly hydrolyzes into two mass spec-friendly, immiscible byproducts.

• Enhanced Enzymatic Digestion: By gently denaturing proteins and breaking up hydrophobic aggregates, RapiGest SF dramatically increases the speed and completeness of proteolytic cleavage, often reducing overnight digestions to just a few hours.

• MS-Compatible Profiling: Because the degraded surfactant byproducts do not ionize efficiently in the positive ion mode, they will not suppress sensitive peptide signals, introduce background noise, or contaminate the mass spectrometer's ion source.

• Eliminates Sample Loss: Bypasses the need for complex, loss-prone desalting or dialysis steps that are traditionally required to scrub detergents like SDS or Triton X-100 out of a sample before HPLC injection.

Product Specifications

• Catalog Number: 186002123

• Product: RapiGest SF Surfactant

• Format: Dry Powder

• Quantity: 10 mg (Per Vial)

• Primary Application: Proteomics / LC-MS Protein Digestion

Key Benefits & Applications

• Shotgun Proteomics: The preferred solubilization agent for complex whole-cell lysates, maximizing the sequence coverage and the total number of identified peptides during bottom-up MS workflows.

• Membrane Protein Solubilization: Highly effective at extracting and maintaining hydrophobic membrane proteins in solution, which are notoriously difficult to digest in purely aqueous buffers.

• High-Throughput Automation: The simple "add, digest, and acidify" workflow avoids physical spin-columns, making it easily adaptable to 96-well plate formats and automated robotic liquid handlers.

• Enzyme Compatibility: Fully compatible with a wide range of proteases, including Trypsin, Lys-C, Asp-N, and Glu-C, without inhibiting their catalytic activity.

Expiration 12-2023

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