Waters UPLC Acquity Column 1.7 um 2.1 x 150 mm

Waters 186004742 ACQUITY UPLC Glycan BEH Amide Column – 1.7 µm, 2.1 x 150 mm

The Waters 186004742 is a premium ACQUITY UPLC column engineered for the high-resolution characterization of complex glycan structures. This column utilizes Hydrophilic Interaction Chromatography (HILIC) driven by an amide-bonded phase, which provides exceptional retention and selectivity for polar glycan species. Built upon Waters' proprietary Ethylene Bridged Hybrid (BEH) particle technology, the column withstands the high pressures of UPLC while maintaining a stable, long-lasting stationary phase. The ultra-small 1.7 µm particle size maximizes peak capacity, enabling the baseline separation of structurally similar neutral and acidic N-linked glycans. With its extended 150 mm length, this column is perfectly suited for detailed structural elucidation and quality control profiling of fluorescently labeled (e.g., 2-AB, 2-AA, or RapiFluor-MS) glycans using fluorescence or high-resolution mass spectrometry (MS) detection.

Technical Performance and Design

• BEH Particle Technology: The Ethylene Bridged Hybrid base particle provides superior mechanical strength and chemical stability compared to traditional silica columns, ensuring long column lifetimes and exceptional batch-to-batch reproducibility.

• Optimized Amide Chemistry: The highly polar amide phase is specifically optimized for HILIC-mode separations, preventing the loss of highly hydrophilic glycans and ensuring sharp, symmetrical peaks.

• Sub-2-Micron Resolution: The 1.7 µm particles drastically reduce diffusion paths, resulting in narrower peaks, higher signal-to-noise ratios, and the ability to resolve complex isobaric glycan structures.

• MS and Fluorescence Compatible: Designed to minimize column bleed, ensuring it is highly compatible with sensitive downstream detection methods, including ESI-MS and standard fluorescence detectors.

Product Specifications

• Catalog Number: 186004742

• Chemistry: Glycan BEH Amide (HILIC)

• Particle Size: 1.7 µm

• Pore Size: 130 Å

• Dimensions: 2.1 mm (Inner Diameter) x 150 mm (Length)

• Max Pressure Rating: 18,000 psi (1,240 bar)

Key Benefits & Applications

• Biotherapeutic Characterization: The gold standard for profiling the N-linked and O-linked glycosylation sites on monoclonal antibodies (mAbs) and recombinant proteins, critical for assessing drug efficacy and safety.

• Structural Isomer Resolution: Provides the extended path length (150 mm) required to fully resolve tricky branched isomers and sialylated species.

• Method Scalability: Offers seamless method transferability within the ACQUITY UPLC family, making it an ideal choice for both upstream process development and downstream quality assurance.

• Robust Lifetime: The chemical stability of the BEH particle minimizes degradation from the high-organic mobile phases typically used in HILIC gradients.

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