Chemglass Media Bottle Assembly 250 ml 2 Port PETG Case of 10 Bottles

Chemglass CLS-1168-0250 Media Bottle Assembly – 250 mL, 2-Port, PETG Square

The Chemglass CLS-1168-0250 is a precision-engineered 250 mL media bottle assembly designed for the sterile transfer of liquids in biopharmaceutical and cell culture applications. This closed-system assembly features a crystal-clear, square PETG (Polyethylene Terephthalate Glycol) bottle for maximum visibility and space-saving storage. The unit is equipped with a 2-port 38-430 mm screw cap, integrated with high-purity C-Flex tubing for fluid path integrity. One port is typically dedicated to fluid aspiration/dispensing, while the second port is fitted with a 0.2 µm hydrophobic vent filter to allow for sterile gas exchange and pressure equalization during liquid transfer. Assembled in a cleanroom environment and supplied sterile, this ready-to-use system eliminates the risks associated with manual bottle capping and open-air media handling.

Technical Performance and Design

• Closed-System Design: Provides a secure, pre-validated fluid path that protects high-value media and buffers from environmental contaminants and airborne pathogens during transfer.

• PETG Square Bottle: The clear PETG construction offers an exceptional gas barrier to maintain pH stability, while the square footprint maximizes density in refrigerators and incubators.

• Integrated Vent Filtration: Features a 25 mm, 0.2 µm PTFE hydrophobic vent filter that prevents vacuum lock during fluid withdrawal and ensures that only sterile air enters the vessel.

• C-Flex Tubing: Equipped with heat-sealable, pump-grade C-Flex tubing, allowing for easy integration into peristaltic pump workflows and secure tube-welding to other sterile bag or bottle systems.

Product Specifications

• Catalog Number: CLS-1168-0250

• Bottle Volume: 250 mL

• Bottle Material: Clear PETG

• Cap Type: 38-430 mm 2-Port Screw Cap

• Tubing Type: C-Flex (Thermoplastic Elastomer)

• Sterility: Gamma Irradiated (Sterile)

Key Benefits & Applications

• Sterile Media Transfer: The premier choice for the aseptic addition of supplements to bioreactors or the sterile harvesting of cell culture supernatants.

• Bioprocess Development: Ideal for small-scale pilot studies where maintaining a validated, closed fluid path is critical for regulatory compliance and experimental reproducibility.

• Ready-to-Use (RTU): Pre-assembled, sterilized, and individually bagged to save laboratory time and reduce the labor costs associated with cleaning and autoclaving traditional glass assemblies.

• Breakage Prevention: The durable PETG construction provides a safer alternative to glass assemblies, especially when transporting precious or hazardous biological materials.

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