Corning Ultra-Low Culture Dish 60 x 15 mm Polystyrene Case of 20 Dishes

Corning® 60 mm x 15 mm Ultra-Low Attachment (ULA) Hydrogel-Coated Culture Dishes, Sterile (3261) – Case of 20

The Corning® 3261 is a highly specialized, research-grade laboratory vessel engineered for the maintenance of primary suspension cultures, tumor spheroids, embryoid bodies, and stem cell aggregates. Precision-molded from high-purity virgin polystyrene, each dish is permanently modified with a covalently bound, hydrophilic, neutrally charged **Ultra-Low Attachment (ULA) hydrogel layer**. This layer minimizes non-specific protein binding, completely preventing anchorage-dependent cell lines from grounding, stretching, or initiating surface-driven differentiation pathways. Supplied as a targeted laboratory case of 20 individually wrapped dishes, this sterile, single-use configuration provides cleanroom aseptic security for cell biology workflows, organoid engineering, and high-purity 3D cellular modeling loops.

Technical Performance & Surface Chemistry

• Covalently Bound Hydrogel Layer: Features a stable, ultra-thin hydrogel matrix bonded to the polystyrene substrate. This neutral barrier suppresses the adsorption of serum proteins, fibronectin, and vitronectin, neutralizing the chemical triggers that drive cell attachment.
• 3D Spheroid and Cluster Optimization: By denying an anchorage plane, cells are forced into a continuous suspension state. This forces natural cell-to-cell signaling loops, promoting the self-assembly of uniform 3D multi-cellular tumor spheroids (MCTS) or embryoid bodies.
• Inverted Optical Clarity Profile: Molded with an optically flat base that ensures high light transmission and low background autofluorescence. This setup allows for distortion-free phase-contrast and high-resolution inverted fluorescence microscopy tracking.
• Aseptic Peel-Pack Isolation: Every dish is pre-sterilized via validated gamma-irradiation loops and individually sealed in a heavy-gauge polymer peel-pouch. This allows operators to easily transfer single units into a biosafety cabinet without compromising cleanroom barriers.

Product Specifications

• Catalog Number / MPN: 3261
• Primary Manufacturer: Corning Life Sciences
• Dish Nominal Scale: 60 mm Nominal Diameter x 15 mm Depth
• Actual Growth Surface Dimensions: $\approx 52.1\text{ mm}$ Actual Base Diameter x $14.2\text{ mm}$ Internal Height
• Surface Chemistry: Ultra-Low Attachment (ULA) Hydrogel Coating (Neutrally charged, non-ionic surface profile)
• Material Composition: Optical-grade, USP Class VI Virgin Polystyrene (PS) | 100% Latex-Free
• Dish Growth Base Area: Nominal 21.3 cm² total base surface area
• Sterility & Purity Standards: Gamma radiation-sterilized (SAL $10^{-6}$ parameter); Certified Non-Pyrogenic and Non-Cytotoxic
• Packaging Configuration: Case of 20 Dishes (Individually bagged units nested securely inside a track-coded master shipping carton)

Key Benefits & Applications

• Tumor Biology & Spheroid Cultivation: Provides the benchmark environment for generating tumor spheroids from primary biopsies, enabling accurate in vitro drug toxicity panels and oncology modeling.
• Stem Cell & Embryoid Body Staging: Prevents early, surface-mediated differentiation of pluripotent stem cells (iPSCs/ESCs), maintaining pluripotency markers during initial multi-lineage expansion or cluster formation.
• Primary Tissue Harvest Maintenance: Serves as an ideal, non-binding pooling basin for fragile neural cells, hepatic clusters, or pancreatic islets immediately following mechanical or enzymatic dissociation.
• **Audit-Ready Inventory Control:** Every sterile blister pouch is directly printed with the catalog number, manufacturing lot code, and expiration metric to streamline compliance tracking inside cGMP-regulated bioprocessing lines.

Corning® is a registered commercial trademark of Corning, Inc. Catalog number 3261 specifies the 60 mm x 15 mm Ultra-Low Attachment hydrogel configuration. For routine attachment-dependent cell cultures or microbial growth applications, companion tissue culture-treated (TC) or native non-treated hydrophobic Petri dish configurations must be integrated into the supply pipeline instead.