{"product_id":"greiner-bio-one-662638-thincert-cell-culture-insert-for-24-well-microplate","title":"Greiner Bio-One Thincert Cell Culture Insert for 24 Well Microplate Case of 48 Inserts and 2 Plates","description":"\u003ch3\u003eThinCert® Cell Culture Inserts for 24-Well Plates, Transparent PET Membrane, 0.4 µm Pore Size (662638) – Case of 48 Inserts\u003c\/h3\u003e\n\u003cp\u003eThe Greiner Bio-One 662638 ThinCert® Insert is a premium, research-grade cell culture consumable designed to establish multi-compartmental tissue models for transport, permeation, co-culture, and polarized monolayer assays. Precision-molded to fit standard 24-well multi-well plates, each insert incorporates a high-purity, transparent polyethylene terephthalate (PET) capillary pore membrane with a 0.4 µm track-etched pore diameter. The membrane surface undergoes a specialized physical vacuum-gas plasma modification to ensure consistent cell attachment and growth. Supplied as a commercial application case of 48 individually blister-wrapped sterile inserts, supplemented with 2 matching multi-well plates, this system is an essential tool for evaluating blood-brain barrier models, drug transport mechanics, and tissue-engineered constructs.\u003c\/p\u003e\n\u003ch3\u003eTechnical Performance \u0026amp; Membrane Architecture\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTrack-Etched PET Membrane Transparency: Fabricated from high-clarity, low-fluorescence polyethylene terephthalate. The 0.4 µm pore size features a defined pore density ($2 \\times 10^6\\text{ pores\/cm}^2$), offering an optimal baseline for diffusion kinetics while physically preventing cell migration between chambers.\u003c\/li\u003e\n\u003cli\u003eAsymmetric Hanging Design Geometry: Engineered with an eccentric, hanging hanger rim configuration. This design positions the insert securely inside the receiver well while creating a dedicated pipette access clearance zone, enabling fluid sampling from the lower (basolateral) compartment without disturbing the insert.\u003c\/li\u003e\n\u003cli\u003eAdvanced TC Surface Activation: The cell-contact membrane faces undergo a uniform tissue culture (TC) plasma treatment. This hydrophilic activation accelerates cell adhesion, focal adhesion point assembly, and confluent monolayer maturation across anchorage-dependent cell lines.\u003c\/li\u003e\n\u003cli\u003eScannable In Vivo Optical Compatibility: The transparent nature of the thin PET membrane permits high-resolution phase-contrast light microscopy, immunofluorescence staging, and transepithelial electrical resistance (TEER) measurement loops directly inside the culture track.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCatalog Number \/ MPN: 662638\u003c\/li\u003e\n\u003cli\u003ePrimary Manufacturer: Greiner Bio-One (ThinCert® Product Portfolio)\u003c\/li\u003e\n\u003cli\u003eCompatible Plate Footprint: 24-Well Microplate Matrix\u003c\/li\u003e\n\u003cli\u003eMembrane Material: High-purity, transparent Polyethylene Terephthalate (PET)\u003c\/li\u003e\n\u003cli\u003ePore Size Diameter: 0.4 µm (Ideal for drug transport, co-culture, and secretion studies)\u003c\/li\u003e\n\u003cli\u003ePore Density Profile: $2 \\times 10^6\\text{ pores\/cm}^2$ nominal concentration\u003c\/li\u003e\n\u003cli\u003eEffective Growth Area: 0.33 cm² per insert unit\u003c\/li\u003e\n\u003cli\u003eRecommended Volume Capacity: Upper Insert (Apical): 0.1 mL to 0.3 mL | Lower Well (Basolateral): 0.6 mL to 1.0 mL\u003c\/li\u003e\n\u003cli\u003eSterility Certification: Gamma-irradiated; Certified Non-Pyrogenic, Non-Cytotoxic, and free of detectable DNase, RNase, and human DNA\u003c\/li\u003e\n\u003cli\u003ePackaging Configuration: Case of 48 Inserts and 2 Plates (Individually blister-sealed sterile inserts grouped inside protective inventory packaging cartons)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003ePolarized Epithelial\/Endothelial Layer Culturing: The standard choice for generating functional cellular barriers—such as Caco-2 intestinal layers or renal tubules—to measure directional compound transport and permeability metrics.\u003c\/li\u003e\n\u003cli\u003eIndirect Cell-to-Cell Co-Culture Paradigms: Enables researchers to culture distinct cell populations in the apical and basolateral compartments simultaneously, facilitating the study of paracrine signaling and secreted factors without direct cell contact.\u003c\/li\u003e\n\u003cli\u003eAir-Liquid Interface (ALI) Tissue Modeling: The rigid, porous PET substrate supports the lifting of epithelial cultures (such as airway or epidermal cells) to the air-liquid interface to promote differentiation and mucus production.\u003c\/li\u003e\n\u003cli\u003e**Universal Multi-Brand Well Fitment:** While supplied with 2 Greiner plates, the physical support ears are geometry-matched to establish a stable hanging mount inside most standard ANSI\/SLAS compliant 24-well microplate configurations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eExpiration Date 2026\/11\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003e\u003csmall\u003eAll product names, logos, and brands are property of their respective owners.\u003c\/small\u003e\u003c\/p\u003e","brand":"Greiner Bio-One","offers":[{"title":"Default Title","offer_id":49261916193020,"sku":"10895","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/greiner-bio-one-thincert-cell-culture-insert-for-24-well-microplate-case-of-48-inserts-and-2-plates-petri-dishesculture-plates-greiner-bio-one-718562.jpg?v=1783549862","url":"https:\/\/lifesciencestrading.com\/zh\/products\/greiner-bio-one-662638-thincert-cell-culture-insert-for-24-well-microplate","provider":"Life Sciences Trading","version":"1.0","type":"link"}