Greiner Bio-One Culture Microplate 96 Well Flat Bottom Sealed Case of 40 Plates

Greiner Bio-One FLUOTRAC™ 600 96-Well Black/µClear® Microplate (655097) – Case of 40

The Greiner Bio-One 655097 is a highly specialized, solid-phase immunoassay microplate engineered for precision high-throughput screening, solid-phase binding assays, and quantitative ELISAs. Part of the advanced FLUOTRAC™ 600 series, these plates feature an opaque black polystyrene chassis designed to eliminate crosstalk, integrated with an ultra-flat, paper-thin µClear® bottom for superior transmission. Supplied pre-sterilized in a high-volume factory case of 40 plates (packed as 10 plates per bag), this high-binding array delivers an increased protein adsorption surface optimized for reliable antigen-antibody immobilization.

Technical Performance & Design

• High-Binding Immunoassay Surface: Specially treated to provide an enhanced protein binding capacity of approx. 600 ng/cm². This modification exhibits high affinity for hydrophilic and hydrophobic biomolecules, making it ideal for standard ELISA development.
• Opaque Black Frame with µClear® Bottom: Formulated with deep black pigments in the well walls to achieve an absolute light barrier that minimizes background autofluorescence and eliminates well-to-well optical cross-talk during top- or bottom-reading assays.
• Isolated Chimney Well Geometry: Designed with an F-bottom chimney well profile where each cylindrical well is structurally separated by a narrow air gap. This layout significantly reduces the risk of capillary fluid bridging and cross-contamination during automated or manual reagent dispatch.
• Exceptional Low-Volume Uniformity: Delivers a highly uniform surface coating with a coefficient of variation (CV) scatter profile of less than 10%, ensuring robust, repeatable baseline determinations across all 96 wells.

Product Specifications

• Model Number / Part No: 655097 (FLUOTRAC™ 600 / µClear® Line)
• Manufacturer: Greiner Bio-One GmbH
• Plate Array Format: Standard 96-Well Matrix (8 x 12 Grid Layout)
• Well Shape & Bottom: Round Chimney Wells with an Ultra-Flat Flat (F) Bottom
• Color Morphology: Solid Black Frame with a Clear Optical Well Base
• Volumetric Capacity: Total Well Volume: 392 µL; Recommended Working Volume: 25 µL to 340 µL
• Sterility Specification: Sterile (Pre-sterilized via validated irradiation protocols)
• Purity Verification: Certified free from detectable RNase, DNase, human DNA, and bacterial endotoxins (Non-pyrogenic)
• Packaging Configuration: Case of 40 Plates (Packaged as 10 plates per bag, 4 bags per case)

Key Benefits & Applications

• Quantitative & Sandwich ELISAs: Provides the stable hydrophobic/hydrophilic balance required for the robust capture of antibodies, viral particles, and complex proteins.
• Bottom-Reading Fluorescence Assays: The ultra-clear µClear® bottom allows bottom-reading fluorometers and high-content imaging systems to achieve optimal signal transmission without signal damping.
• Standardized Automation Footprint: Complies fully with universal ANSI/SLAS 1-2004 microplate footprint dimensions, facilitating seamless interface positioning with automated liquid handlers, plate stackers, and barcode washers.
• Alphanumeric Matrix Indexing: Features crisp, molded alphanumeric side markers (A1 through H12) combined with a distinct corner notch to guarantee correct directional orientation and prevent micro-volume tracking errors.

Expiration Date 2021-07

Greiner Bio-One®, FLUOTRAC™, and µClear® are registered trademarks of Greiner Bio-One GmbH. The 655097 catalog code represents the high-binding, sterile black/clear bottom configuration, which ships from the factory without lids. If your storage or incubation loops require evaporation barriers, matching Greiner lids or silicone CapMats must be specified independently.