Greiner Bio-One Culture Microplate w Lid 96 Well Sterile Case of 32 Plates

Greiner Bio-One CELLSTAR 96-Well Black/µClear® Microplate with Black Lid (655090-99) – Case of 32

The Greiner Bio-One 655090-99 is a highly specialized, automation-compatible 96-well tissue culture microplate engineered for fluorescence, luminescence, and high-content screening (HCS) of light-sensitive biological samples. Building upon the standard optical-bottom chimney well architecture, this model features a solid black polystyrene frame with an ultra-flat, paper-thin µClear® film bottom for excellent bottom-reading transmission. Uniquely supplied with a **matching opaque black lid** to completely isolate specimens from ambient room light, this tissue culture (TC) treated array comes sterile and vacuum-sealed in a facility case of 32 plates.

Technical Performance & Design

• Opaque Black Frame with µClear® Bottom: Formulated with deep black pigments inside the vertical well walls to block light transmission, preventing well-to-well optical cross-talk and scattering during sensitive bottom-reading fluorometric assays.
• Light-Blocking Black Lid Assembly: Outfitted with a specialized, solid black polystyrene lid that provides absolute protection against ambient light degradation, making it highly optimized for long-term cell incubation of light-sensitive compounds or fluorophores.
• Vacuum-Gas Plasma TC Surface Treatment: Enhanced via a proprietary physical surface modification process that introduces hydrophilic polar functional groups to promote robust, uniform attachment and spreading of adherent cell lines.
• Isolated Chimney Well Geometry: Utilizes an F-bottom chimney well configuration where individual well profiles are structurally separated by an air gap, eliminating capillary fluid bridges and minimizing cross-contamination during automation cycles.

Product Specifications

• Model Number / Part No: 655090-99 (CELLSTAR® / µClear® Light Protection Line)
• Manufacturer: Greiner Bio-One GmbH
• Plate Configuration: Standard 96-Well Matrix (8 x 12 Grid Array)
• Well Shape & Bottom: Round Chimney Wells with an Ultra-Flat Flat (F) Film Bottom
• Color Architecture: Solid Black Housing Frame with a Clear Optical Well Floor
• Lid Configuration: High-Density Opaque Black Protective Lid
• Total Well Capacity: 392 µL (Recommended operational working volume: 25 µL to 340 µL)
• Sterility Status: Sterile (Pre-sterilized via validated beta irradiation protocols)
• Purity Verification: Certified free from detectable RNase, DNase, human DNA, and bacterial endotoxins (Non-pyrogenic)
• Packaging Layout: Case of 32 Plates (Packaged as 8 plates per pack, 4 packs per case)

Key Benefits & Applications

• Light-Sensitive Cell-Based Assays: Essential for tracking fluorescent proteins, photogenic reporter systems, or testing compounds that decompose rapidly upon ambient light exposure.
• High-Magnification Live-Cell Imaging: The ultra-thin µClear® baseline allows microscopic objectives to achieve sharp focal plane registration, making it ideal for automated confocal and phase-contrast screening platforms.
• Standardized Automation Footprint: Adheres strictly to universal ANSI/SLAS footprint dimensions, ensuring precise mechanical alignment with robotic grippers, liquid handling decks, and microplate readers.
• Alphanumeric Identification Matrix: Features a high-contrast molded alphanumeric grid index (A1 through H12) paired with a distinct notched corner to guarantee precise plate orientation and eliminate loading errors.

Greiner Bio-One®, CELLSTAR®, and µClear® are registered trademarks of Greiner Bio-One GmbH. The 655090-99 part number designates the light-protected format bundled with a black lid. If your protocol requires a standard clear lid for continuous transmission checks, the standard [Greiner Bio-One 655090 Microplate](http://googleusercontent.com/shopping_content/49_link) option should be sourced instead.