Invitrogen MagicMedia E Coli Expression Medium Total of 4

Invitrogen™ MagicMedia™ E. coli Expression Medium – Ready-to-Use Liquid (MPN: K6815)

The Invitrogen™ (Thermo Fisher Scientific) K6815 MagicMedia™ E. coli Expression Medium is a high-yield, auto-induction liquid culture medium specially formulated for the high-density growth and recombinant protein expression of T7 promoter-based E. coli systems (such as pET vectors in BL21 derivatives). Designed to eliminate the need for optical density monitoring and manual IPTG induction, this ready-to-use medium automatically triggers protein synthesis upon reaching optimal cell density, yielding up to 10-fold higher target protein concentrations compared to standard LB broth.

• Proprietary Auto-Induction Formulation: Engineered with a balanced combination of carbon and energy sources that selectively regulates the lac operon, suppressing recombinant protein expression during early log-phase growth and automatically inducing high-level expression as glucose is depleted.
• Hands-Free Workflow Optimization: Completely eliminates the labor-intensive process of monitoring culture OD600 levels and manually adding IPTG, allowing researchers to set up overnight or weekend expression runs with minimal hands-on intervention.
• High Cell Density & Protein Yields: Delivers significantly higher cell densities and recombinant protein yields—often achieving 3 to 10 times the volumetric protein output of conventional Luria-Bertani (LB) or Terrific Broth (TB) protocols.
• Reduced Toxicity & Basal Expression: Tight metabolic control prevents premature expression of potentially toxic target proteins during early growth phases, preserving culture viability and maximizing final protein integrity.
• Ready-to-Use Liquid Format: Supplied as a pre-mixed, sterile-filtered liquid formulation for immediate inoculation without requiring powder reconstitution, autoclaving, or supplement preparation.
• Broad System Compatibility: Fully compatible with standard T7-regulated expression hosts (including BL21(DE3), Rosetta™, and Dual Media strains) cultured in shake flasks, deep-well blocks, or automated bioreactor platforms.