Invitrogen Neon Transfection System with Pipette Station

Invitrogen Neon Transfection System (MPK5000) with Pipette Station

The Invitrogen Neon Transfection System (MPK5000) is a benchtop electroporation device designed for the highly efficient delivery of nucleic acids, proteins, and siRNA into a wide range of difficult-to-transfect cells. By utilizing a proprietary biological pipette tip as the transfection chamber, the system minimizes cell toxicity by providing a more uniform electric field compared to traditional cuvette-based methods. This versatile platform is a staple in research and bioproduction labs for applications ranging from CRISPR/Cas9 gene editing to primary cell analysis.

Technical Performance & Design

• Proprietary Pipette Tip Technology: Replaces standard electroporation cuvettes with a gold-plated electrode tip, significantly increasing the surface-area-to-volume ratio for better cell viability.
• Uniform Electric Field: The unique design reduces pH changes, temperature fluctuations, and metal ion release, ensuring higher recovery rates even in sensitive primary cells and stem cells.
• Integrated Pipette Station: The MPK5000 includes a dedicated station that acts as the high-voltage connection point, allowing for seamless liquid handling and "plug-and-play" transfection.
• Simplified Optimization: Features a user-friendly touchscreen interface with pre-programmed protocols for over 140 cell types, reducing the time required for experimental scale-up.

Product Specifications

• Catalog Number: MPK5000
• Compatible Formats: 10 µL and 100 µL Neon Tips
• Input Voltage: 100–240 VAC
• Dimensions: 9.2" (W) x 11.8" (D) x 8.6" (H)
• Pulse Voltage Range: 500–2,500 V
• Throughput: 1 sample per 15–30 seconds

Key Benefits & Applications

• High Transfection Efficiency: Achieve superior results in "hard-to-transfect" lines, including Jurkat, HEK-293, and neonatal keratinocytes.
• Maximum Cell Viability: Gentler electroporation parameters preserve cell health, making it ideal for downstream functional assays and phenotypic screening.
• Streamlined Workflow: The "Pipette, Zap, Plate" process eliminates the need for multiple transfer steps, reducing the risk of contamination and sample loss.
• Versatile Cargo Delivery: Specifically engineered to handle various payloads including DNA, RNA, and RNP complexes for advanced gene-editing workflows.

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