Milenyi Biotec AutoMACS Column Sterile Pack of 8 Columns

Miltenyi Biotec® autoMACS® Columns, Sterile (130-021-101) – Pack of 8

The Miltenyi Biotec® 130-021-101 is a specialized, high-performance automated magnetic cell separation consumable engineered exclusively for use with the autoMACS® and autoMACS® Pro Automated Cell Separator instruments. Utilizing an innovative hydrophilic matrix of ferromagnetic spheres, these columns generate an ultra-intense, localized magnetic gradient when positioned within the instrument's magnetic field block. This design enables the high-purity isolation of magnetically labeled target cell populations. Supplied pre-sterilized as a factory pack of 8 columns, this single-use consumable guarantees exceptional viability and high recovery rates across automated sorting runs.

Technical Performance & Design

• High-Gradient Ferromagnetic Matrix: Packed with precision-engineered, biocompatible steel spheres coated in a specialized synthetic polymer layer. When the automated separator activates its external magnetic field, this matrix amplifies the magnetic forces to capture micro-sized MACS® MicroBeads cleanly without crushing or stressing the cell bodies.
• Automated High-Throughput Processing: Specifically engineered to fit the mechanical fluid paths and valve manifolds of the autoMACS® series. This integration enables fully automated multi-sample cell sorting loops that run safely without manual oversight.
• Optimized Hydrodynamic Flow Profiles: The uniform spacing between the matrix spheres allows unlabelled cells and structural debris to pass through freely while preventing nonspecific trapping, preserving exceptional downstream target cell purity.
• Pre-Sterilized and Safe: Individually packed and sterilized using validated methods. This ensures the fluid paths remain completely free of external bio-burden, keeping primary tissue collections and downstream cell lines safe during isolation.

Product Specifications

• Model Number / Catalog No: 130-021-101 (autoMACS® Consumable Series)
• Primary Manufacturer: Miltenyi Biotec® Inc.
• Instrument Compatibility: Benchtop autoMACS® and autoMACS® Pro Separator units
• Separation Surface Chemistry: Non-toxic, hydrophilic polymer-coated ferromagnetic sphere bedding
• Sterility Baseline: Certified Pre-Sterilized via radiation parameters
• Purity Verification: Non-pyrogenic, endotoxin-tested, and optimized to keep delicate primary cells highly viable
• Maximum Cell Sample Load: Optimized to process up to $2 \times 10^8$ magnetically labeled cells or $2 \times 10^9$ total cell inputs per automated column run
• Packaging Configuration: Pack of 8 Columns (Each column is safely sealed within a sterile convenience pouch)

Key Benefits & Applications

• Positive Target Selection: Ideal for isolating rare target populations, such as specific stem cell subsets, CD4+/CD8+ T-lymphocytes, or dendritic cells, retaining them inside the matrix until demagnetization triggers elution.
• Depletion Sorting Routines: Highly effective at wiping away unwanted background cells—such as removing dead components or non-target erythrocytes—to yield a highly purified, unlabelled target fraction in the flow-through.
• Oncology Stem Cell Isolation: Frequently integrated into cancer research tracks to isolate tumor-initiating cells or rare circulating tumor cells (CTCs) from dense tissue extractions or whole blood matrices.
• Downstream Genomic Validation: Delivering high-purity cells ensures clean results for downstream single-cell RNA sequencing (scRNA-seq), PCR analysis, flow cytometry profiling, or adoptive cell transfer assays.

Miltenyi Biotec®, MACS®, and autoMACS® are registered commercial trademarks of Miltenyi Biotec B.V. & Co. KG. Catalog number 130-021-101 specifies the automated sterile column pack. For manual separation procedures utilizing static magnets, separate gravity-driven LS, MS, or LD column arrays must be substituted.