Repligen MidiKros Hollow Fiber Filter 20 cm Length 0.2 um PES 1 mm FLL X FLL

Repligen MidiKros Hollow Fiber Filter, 20 cm Length, 0.2 µm mPES, 1.0 mm ID, FLL x FLL (P-D02-E20U-10-N)

The Repligen MidiKros Hollow Fiber Filter (P-D02-E20U-10-N) is a high-performance single-use tangential flow filtration (TFF) module engineered for pilot-scale, benchtop, and clinical bioprocessing applications. Featuring a 0.2 µm sterilizing-grade pore size microfiltration membrane composed of modified Polyethersulfone (mPES), this filter delivers exceptional flux rates, low protein binding, and high volumetric throughput during bio-burden reduction, cell clarification, and particle separation workflows. Designed with a 20 cm active path length and a wide 1.0 mm lumen inner diameter (ID) fiber geometry, the module reduces fluid shear stress and accommodates higher viscosity fluids or higher cell density feeds. Encapsulated in a clear, biocompatible polysulfone housing with standard Female Luer Lock (FLL) process and permeate port connections, the MidiKros module seamlessly integrates into automated or manual TFF assemblies, enabling linear scale-up to larger bioprocess-scale filtration systems while eliminating cleaning validation and cross-contamination risks.

Technical Performance & Structural Attributes

• Advanced mPES Membrane Chemistry: Hydrophilized modified Polyethersulfone provides minimal non-specific binding, excellent chemical tolerance across a broad pH range, and reduced membrane fouling during complex biological separations.
• Wide 1.0 mm Lumen Diameter: High-clearance 1.0 mm inner diameter fibers reduce cross-flow shear stress on delicate cellular structures while preventing channel clogging from high-solids feeds.
• Single-Use Encapsulated Design: Factory-assembled and potted in a USP Class VI housing, eliminating cartridge installation steps, system cleaning validation, and water-for-injection (WFI) pre-flushing requirements.
• Standardized Luer Lock Interface: Features Female Luer Lock (FLL) fittings on both process feed/retentate flow paths and side permeate ports for fast, leak-free connection to flexible tubing circuits and sampling manifolds.
• Linear Bioprocess Scalability: Shares identical fluid channel height and membrane properties with larger KrosFlo and MaxiKros hollow fiber modules to ensure predictable performance during scale-up.

Product Specifications

• Catalog / Part Number: P-D02-E20U-10-N
• Primary Manufacturer: Repligen Corporation
• Filter Module Series: MidiKros Hollow Fiber Module
• Membrane Material: Modified Polyethersulfone (mPES)
• Pore Size Rating: 0.2 µm (Microfiltration / Bio-burden Reduction)
• Fiber Inner Diameter (ID): 1.0 mm
• Active Module Path Length: 20 cm
• Feed / Retentate Connections: Female Luer Lock (FLL)
• Permeate Port Connections: Female Luer Lock (FLL)
• Housing Material: Polysulfone (PSU), USP Class VI Compliant
• Regulatory Compliance: USP Class VI, ISO 10993, Gamma Irradiated / Autoclavable, Non-Pyrogenic

Key Benefits & Applications

• Sterilizing Microfiltration & Bio-Burden Reduction: Provides effective removal of bacteria, cellular fragments, and particulate matter from culture broths and buffer solutions.
• Cell Clarification & Bioreactor Harvesting: Ideal for continuous or batch harvesting of mammalian cells (CHO, HEK293) and plant cell cultures under low-shear fluidic conditions.
• Viral Vector & Nanoparticle Concentration: Gently concentrates large biological complexes, adenovirus/AAV vectors, liposomes, and cellular exosomes without altering particle integrity.

All product names, logos, and brands are property of their respective owners. Repligen, MidiKros, KrosFlo, and MaxiKros are registered trademarks of Repligen Corporation.