Shimadzu Column C18 Nexcol Test Mix 5 um 50 x 3.0 mm

The Shimadzu 220-91394-02 is a Nexcol C18 HPLC Column specifically designed for high-performance liquid chromatography. This column is primarily used for Hardware Validation (IQ/OQ). It is used with a Test Mix to verify the system's flow rate, gradient accuracy, and detector sensitivity during installation or annual maintenance. The 3.0 mm ID provides a middle ground between standard 4.6 mm analytical columns and 2.1 mm microbore columns, offering improved sensitivity with lower solvent consumption while maintaining manageable back pressures. In environmental labs, this column is frequently utilized as a Delay Column for PFAS (Per- and Polyfluoroalkyl Substances) analysis. It is installed before the injector to separate background PFAS contaminants originating from the LC system itself from the analytes in the sample.