Tecan HydroFlex Elisa Microplate Washer

The Tecan 30087532 HydroFlex is a modular and highly flexible microplate washer designed for automated ELISA assays, cell washing, and bead-based applications. As a cornerstone of the HydroFlex series, this unit is recognized for its exceptional reliability in clinical and research laboratories. Its modular architecture allows for seamless transitions between standard 96-well ELISA protocols and specialized cell or bead-washing tasks, providing a versatile solution for high-throughput plate processing.

Technical Performance and Design

• Precision Manifold Design: Features high-accuracy dispensing and aspiration pins engineered to provide uniform fluid distribution across all 96 wells simultaneously.

• Crosswise Aspiration Technology: Optimized for flat-bottom plates to achieve ultra-low residual volumes (<2 µl per well), ensuring thorough cleaning and reducing background noise in sensitive assays.

• Advanced Fluid Monitoring: Equipped with integrated liquid level sensors to prevent overflows and alert users to low buffer levels during automated runs.

• Programmable Wash Cycles: Supports complex multi-step protocols with adjustable soak times, agitation speeds, and volume settings via an intuitive onboard interface.

Product Specifications

• Part Number: 30087532

• Plate Compatibility: Standard 96-well formats (flat, V, and U-bottom)

• Residual Volume: <2 µl per well (for 96-well flat-bottom plates)

• Dispense Accuracy: <2% CV across the plate

• Interface: Onboard keypad with multi-line LCD display and PC-link capability

• Modular Options: Supports Magnetic Bead Separation (MBS) and Vacuum Filtration modules

Specialized Application Support

• ELISA Washing: Optimized for high-throughput diagnostic workflows, providing consistent washing efficiency for protein-binding assays.

• Gentle Cell Washing: Features adjustable aspiration/dispense speeds and a specialized "drop-wise" mode to minimize shear force, protecting adherent or weakly adherent cell lines.

• Magnetic Bead Processing: Compatible with neodymium MBS plate carriers (two magnets per well) for efficient settling of magnetic microparticles.

• Vacuum Filtration: Integrated vacuum sensors support automated DNA purification and non-magnetic bead assays through programmable filtration steps.

Operational Advantages

• Minimized Cross-Contamination: The non-contact dispensing system and automated rinse protocols maintain the highest levels of aseptic integrity between plates.

• Rapid Protocol Switching: The modular carrier system allows users to swap between magnetic, filtration, and standard washing manifolds in seconds.

• Compact Footprint: Designed for space-constrained laboratory benches while maintaining full industrial-scale washing capabilities.

• Robust Self-Maintenance: Features automated "prime" and "rinse" routines to prevent salt crystal buildup in the manifold pins, extending the life of the fluidics path.

Used; excellent condition. See working video here.