{"product_id":"tecan-microplate-reader-430-nm-filter-for-genios-series","title":"Tecan Microplate Reader 430 nm Filter for GENios Series","description":"\u003cp\u003e\u003cspan class=\"citation-160\"\u003eThe 430 nm filter for the Tecan GENios series is a specialized optical component used primarily for absorbance measurements and specific fluorescence excitation protocols. In the GENios architecture, these filters are mounted into specialized excitation or emission \u003c\/span\u003e\u003cspan class=\"citation-160\"\u003efilter slides\u003c\/span\u003e\u003cspan class=\"citation-160 citation-end-160\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 data-path-to-node=\"1\"\u003eCore Applications\u003c\/h4\u003e\n\u003cp data-path-to-node=\"2\"\u003eThe 430 nm wavelength is a critical \"cross-over\" point for several advanced biochemical assays:\u003c\/p\u003e\n\u003cul data-path-to-node=\"3\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"3,0,0\"\u003eBRET (Bioluminescence Resonance Energy Transfer): This is one of the most common uses for a 430 nm filter. It acts as the donor emission filter for assays using \u003ci data-path-to-node=\"3,0,0\" data-index-in-node=\"161\"\u003eRenilla\u003c\/i\u003e Luciferase (RLuc). RLuc reacts with coelenterazine to emit blue light centered around 430–480 nm, which then excites a nearby acceptor (like YFP).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"3,1,0\"\u003eEnzymatic Activity (Arginase): In absorbance mode, 430 nm is specifically used to measure arginase activity, where the reaction product reacts with a substrate to produce a color change proportional to enzyme activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"3,2,0\"\u003eFluorescence Emission (NADH\/NADPH): While the peak emission for NADH is typically 450 nm, 430 nm filters are often used to capture the lower end of the blue fluorescence spectrum when monitoring metabolic states or cellular respiration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"3,3,0\"\u003eReporter Gene Assays: Used in dual-color luminescence or fluorescence assays (like Chroma-Luc technology) to separate violet\/blue signals from longer-wavelength yellow or green signals.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"citation-159\"\u003eUsed; excellent condition.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Tecan","offers":[{"title":"Default Title","offer_id":48983918412028,"sku":"10656","price":190.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/tecan-microplate-reader-430-nm-filter-for-genios-series-filters-tecan-390361.jpg?v=1776500174","url":"https:\/\/lifesciencestrading.com\/zh\/products\/tecan-microplate-reader-430-nm-filter-for-genios-series","provider":"Life Sciences Trading","version":"1.0","type":"link"}