Thermo LabSystems Luminoskan Ascent Microplate Reader with Ascent Software 2.6

Thermo LabSystems Luminoskan Ascent Microplate Luminometer (5300170) with Ascent Software 2.6

The Thermo LabSystems 5300170 Luminoskan Ascent is a high-performance, dedicated microplate luminometer engineered for precise, high-sensitivity luminometric research applications. Factory-configured with one integrated reagent dispenser and paired with Ascent Software v2.6, this system provides the rapid measurement speeds and wide dynamic range required for critical flash and glow assays. Its fiberless direct illumination optics support both top and bottom reading, ensuring minimal well-to-well crosstalk and exceptional data precision in busy research and biotechnology laboratories.

Technical Performance & Design

• Integrated Reagent Dispensing: Features one onboard, high-precision reagent dispenser capable of simultaneous dispensing and reading. This enables accurate monitoring of rapid kinetic assays from the exact moment the reaction begins.
• Fiberless Direct Optics: Utilizes a direct-illumination optical path for both top and bottom reading, maximizing light collection efficiency and expanding the dynamic range while minimizing background interference.
• Fast Plate Processing: Delivers rapid data acquisition cycles, completing a full 96-well plate scan in as few as 15 seconds, which is essential for high-throughput screening and time-sensitive kinetic profiles.
• Low Dead-Volume Fluidics: Designed with an optimized dispenser tip path featuring a backflush capability, reducing valuable reagent waste when priming or purging lines between assay groups.

Product Specifications

• Model / Part Number: 5300170 (Luminoskan Ascent Type 392 Array)
• Measurement Mode: Dedicated Luminometry (DLReady™ certified by Promega)
• Plate Formats: Accommodates standard SBS-compliant 6-, 12-, 24-, 48-, 96-, and 384-well microplates
• Dispenser Configuration: Includes 1 Onboard Dispenser (Upgradeable up to 3 total)
• Dispensing Volume Range: 1 to 1000 µL, adjustable in 1 µL increments
• Spectral Selection: Internal filter wheel accommodates up to 6 independent emission filters
• Control Software: Ascent Software Version 2.6 (PC-compatible protocol builder and data exporter)
• Electrical Architecture: 100V - 240V AC, 50/60 Hz (Universal auto-switching line)

Key Benefits & Applications

• Reporter Gene & Luciferase Assays: Provides the high sensitivity and instant dispensing required for dual-luciferase reporter assays and gene regulation profiling.
• ATP & Cell Viability Quantification: Optimizes the rapid tracking of low-level ATP luminescent signals during high-throughput cytotoxicity and cell proliferation indexing.
• Luminescent Immunoassays (LIA): Ensures tight signal-to-noise ratios for chemiluminescent immunoassays, providing reliable trace target detection in serum or cellular lysates.
• Ascent Software Control Panel: Version 2.6 allows laboratory operators to easily design complex kinetic loops, define integration times, and automate background subtraction calculations for GLP-compliant workflows.

Used, tested to be in great working condition. See starting up video here.

Luminoskan Ascent® and Ascent Software® are registered trademarks of Thermo Electron Corporation (Thermo Fisher Scientific). For optimal luminometric signal capture and to prevent well-to-well optical crosstalk, the use of solid white microplates is highly recommended.