Thermo Scientific Streptavidin Microplate 96 Well Box of 5 Plates

Thermo Scientific Pierce Streptavidin-Coated 96-Well Microplate, Clear (15502) – Box of 5 Plates

The Thermo Scientific Pierce Streptavidin-Coated Microplate (15502) is a high-binding affinity plate designed for immobilizing biotinylated peptides, proteins, nucleic acids, and antibodies in high-throughput enzyme-linked immunosorbent assays (ELISA), binding assays, and screening applications. Manufactured from medical-grade clear polystyrene, each 96-well plate is pre-coated with highly purified recombinant streptavidin and pre-blocked with a proprietary blocker to minimize non-specific binding and ensure high signal-to-noise ratios. Featuring a high biotin-binding capacity of ≥5 pmol biotin per well, these pre-coated microplates eliminate the need for manual, multi-step passive coating procedures, offering superior well-to-well uniformity and batch-to-batch reproducibility across critical bioanalytical workflows.

Technical Performance & Structural Attributes

• High Biotin-Binding Capacity: Pre-coated streptavidin layer binds biotinylated ligands rapidly and stably, providing high capture efficiency for small molecules, oligonucleotides, and proteins.
• Pre-Blocked Surface Matrix: Factory-treated with a specially formulated blocking buffer to eliminate background non-specific adsorption without interfering with specific binding events.
• High-Optical-Clarity Clear Polystyrene: Clear flat-bottom wells deliver optimal light transmission for visual, colorimetric (absorbance), and spectrophotometric assay readouts.
• Standard ANSI/SLAS Geometry: Standardized microplate footprint ensures full compatibility with automated liquid handlers, microplate washers, and standard plate readers.
• Consistent Coating Uniformity: Stringent quality control yields low well-to-well variation (%CV) for high quantitative accuracy in diagnostic and assay development routines.

Product Specifications

• Catalog / Part Number: 15502
• Primary Manufacturer: Thermo Scientific (Thermo Fisher Scientific / Pierce Biotechnology)
• Plate Format: 96-Well Standard Microplate
• Well Bottom Geometry: Flat Bottom
• Plate Color / Clarity: Clear
• Coating Surface: Recombinant Streptavidin (Pre-blocked)
• Biotin-Binding Capacity: ≥5 pmol biotin per well
• Material Composition: Polystyrene (PS)
• Sterility Status: Non-Sterile
• Packaging Configuration: Box of 5 Individually Wrapped Plates

Key Benefits & Applications

• Biotinylated Antibody & Antigen Capture: Ideal for capturing biotinylated antibodies, recombinant proteins, and antigens in sandwich and competitive ELISAs.
• Hybridization & Oligonucleotide Assays: Efficiently immobilizes biotinylated DNA/RNA probes and PCR products for nucleic acid hybridization assays.
• Streamlined Assay Workflows: Pre-coated and pre-blocked format reduces assay preparation time and eliminates variability associated with manual coating protocols.

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