Thermo Scientific Rapid Flow Filter 250 ml 0.2 um PES Case of 12 Filters

Thermo Scientific™ Nalgene™ Rapid-Flow™ Sterile 250 mL Vacuum Filter Units, 0.2 µm PES (568-0020) – Case of 12

The Thermo Scientific™ Nalgene™ 568-0020 is a complete, single-use vacuum filtration system engineered for the sterile purification and storage of mammalian cell culture media, fetal bovine serum (FBS), sensitive biological reagents, and analytical buffers. This full assembly consists of a 250 mL upper filter funnel pre-docked to a matching 250 mL receiver storage bottle. Utilizing Nalgene’s proprietary Rapid-Flow™ multi-column support plate system paired with a high-performance, asymmetric polyethersulfone (PES) membrane, it delivers maximum flow rates and excellent throughput while providing a certified 0.2 micrometer (µm) absolute biological contamination barrier. Supplied as a commercial facility case of 12 individually wrapped units, this model is a core consumable for benchtop research, upstream bioprocessing scale-up, and cleanroom fluid management.

Technical Performance & Membrane Architecture

• Asymmetric Hydrophilic PES Membrane: Features a 50 mm polyethersulfone membrane with an asymmetric pore configuration. The upper surface has larger pores that act as an integrated pre-filter to catch macro-particulates, while the tighter 0.2 µm bottom layer ensures absolute microbial retention, reducing processing downtime caused by premature membrane clogging.
• Ultra-Low Protein Binding Baseline: Specially selected to prevent the non-specific binding of critical growth factors, enzymes, and proteins. This ensures that premium serum supplements and media additives retain their exact chemical and nutritional balance post-filtration.
• Proprietary Rapid-Flow™ Support System: The internal support infrastructure underneath the membrane optimizes vacuum pressure distribution. This minimizes structural stress on the PES matrix, prevents premature clogging, and reduces overall filtration time under a biosafety hood.
• Leak-Proof Receiver Storage: The lower 250 mL receiver storage bottle uncouples easily from the filter collar and seals airtight with an included 1.5-turn high-density polyethylene (HDPE) screw cap. This secure seal protects filtered reagents from atmospheric exposure and prevents evaporation-driven pH shifts during cold room storage.

Product Specifications

• Catalog Number / MPN: 568-0020 (Alternative Ref: 5680020)
• Primary Manufacturer: Thermo Scientific™ (Nalgene™ Filtration Portfolio)
• Volumetric Capacity: 250 mL Upper Funnel / 250 mL Lower Receiver Storage Bottle
• Membrane Pore Size: 0.2 µm absolute sterilizing grade
• Membrane Diameter Profile: 50 mm nominal diameter
• Membrane Material Chemistry: Hydrophilic Asymmetric Polyethersulfone (PES)
• Housing Material Composition: Crystal-clear, impact-resistant polystyrene (PS) with a blue identifying collar
• Sterility & Purity Standards: Gamma radiation-sterilized; Certified Non-Pyrogenic, Non-Cytotoxic, and low-extractable
• Packaging Configuration: Case of 12 Complete Units (Each unit individually wrapped in an easy-to-peel aseptic pouch, complete with standalone storage screw closures)

Key Benefits & Applications

• Upstream Mammalian Media Preparation: The premier choice for fast serialization of fragile base media, fetal bovine serum (FBS) blends, antibiotics, and vitamin supplements where high protein recovery is paramount.
• High-Purity Molecular Master Mix Staging: Provides an inert, contaminant-free pathway for filtering complex salt matrices, mobile phases, and HPLC buffers without leaching trace chemical plasticizers into the analytical line.
• Ergonomic and Safe Vacuum Hookups: Features an integrated, universal quick-connect vacuum tubing adapter that ensures a stable, leak-proof line connection, reducing benchtop clutter and accidental tipover risks.
• **cgmp-Compliant Lot Traceability:** Every individual filter body is clearly printed with the catalog number, manufacturing lot code, membrane material, and pore size specification to ensure seamless facility traceability.

Thermo Scientific™, Nalgene™, and Rapid-Flow™ are registered commercial trademarks of Thermo Fisher Scientific Inc. Catalog number 568-0020 specifies the 250 mL complete filter assembly with a 0.2 µm PES membrane. For filtering larger volumes or using aggressive organic solvents that require alternative membranes like Nylon or Cellulose Acetate, larger 500 mL/1000 mL profiles or alternative polymer configurations should be selected.