Phenomenex Strata-X Microplate 2 mg Polymeric Reversed Phase

Phenomenex Strata-X Microelution SPE Plate – 2 mg/well, Reversed Phase, 96-Well

The Phenomenex 8M-S100-4GA is a high-performance Solid Phase Extraction (SPE) microplate engineered for the rapid, high-throughput isolation of a broad spectrum of target analytes from ultra-low volume biological samples. Configured in a specialized microelution format, each well in the 96-well manifold contains a minimal 2 mg bed of Strata-X, a 33 µm polymeric reversed-phase sorbent. Unlike traditional silica-based C18 sorbents, this rugged polymeric backbone resists deconditioning if accidentally dried out during the conditioning or washing steps, entirely eliminating the risk of lost sample recoveries. The microelution design enables analyte elution in volumes as low as 25 µL, completely bypassing the nitrogen dry-down step that frequently causes adsorptive losses of "sticky" analytes to the walls of collection plates. This results in ultra-clean, highly concentrated extracts that are immediately ready for LC-MS/MS analysis.

Technical Performance and Design

• Microelution Efficiency (2 mg): The ultra-low 2 mg sorbent mass is explicitly optimized for micro-volume biological samples. It allows for highly concentrated elution volumes (25–50 µL), drastically reducing processing time by completely eliminating the need for solvent evaporation and reconstitution.

• Universal Retention Mechanisms: The specially modified styrene divinylbenzene polymer offers three mechanisms of retention (pi-pi, hydrogen bonding, and hydrophobic interactions), tightly retaining a massive variety of neutral, basic, and acidic compounds.

• Resistant to Drying: The advanced polymeric backbone prevents the sorbent from collapsing, ensuring that accidental drying under a vacuum manifold will not compromise sensitive extraction recoveries or waste valuable samples.

• Direct-to-Vial Workflow: The concentrated extracts can be eluted directly into an analytical collection plate or autosampler vial insert, streamlining the transition from sample preparation directly to the mass spectrometer.

Product Specifications

• Catalog Number: 8M-S100-4GA

• Format: 96-Well Microelution Plate

• Phase/Chemistry: Strata-X (Polymeric Reversed Phase)

• Particle Size: 33 µm (85 Å Pore Size)

• Sorbent Mass: 2 mg per well

• Target Analytes: Broad Spectrum (Neutrals, Acids, and Bases)

Key Benefits & Applications

• Low-Volume Sample Extraction: The absolute gold standard for pediatric testing, murine (mouse) models, tears, dried blood spots, or any diagnostic assay where the available biological sample volume is strictly limited.

• Eliminates Evaporation Losses: Bypassing the dry-down step is critical for volatile compounds or hydrophobic peptides that frequently stick irreversibly to the plastic walls of collection plates during solvent evaporation.

• LC-MS/MS Optimization: Highly effective at stripping away phospholipids, salts, and particulates, preventing the ion-suppression effects that frequently plague sensitive mass spectrometry detectors.

• High-Throughput Automation: The standard SBS footprint is perfectly optimized for automated liquid handling robots, positive pressure manifolds, and rapid batch processing without manual intervention.

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